. 2020 Oct 31;16(4):543–559. doi: 10.1007/s11302-020-09732-z

Table 5.

Affinities of AST-004, 2-methylthioadenosine, or NECA at adenosine receptors determined in radioligand binding studies

	Human A₃AR	Rat A₃AR	Mouse A₃AR	Human A₁AR	Rat A₁AR	Mouse A₁AR
Agonist	K_i ± SEM (nM)
AST-004	1490 ± 410	10,800 ± 1200	4940 ± 974	1590 ± 853	1880 ± 507	3690 ± 877
2-Methylthio-adenosine^b	720 ± 280	-	-	1900 ± 770	-	-
NECA	6.18 (K_D) ^a	48.6 (K_D)^a	15.1 (K_D) ^a	14^a	5.1^a	2.49^a

	Human A_2AAR	Rat A_2AAR	Mouse A_2AAR	Human A_2BAR	Rat A_2BAR	Mouse A_2BAR
Agonist	K_i ± SEM (nM)
AST-004	16,000 ± 6170	10,200 ± 1990	9040 ± 4290	> 10,000	> 10,000	10,000
2-Methylthio-adenosine^b	11,600 ± 1000	-	-	> 30,000	-	-
NECA	20^a	9.7^a	43.4^a	1890^a	1110^a	656^a

^aData from Alnouri et al. (2015)

^bMethods from Gao et al. (2003)