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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Methods. 2020 Aug 2;187:13–27. doi: 10.1016/j.ymeth.2020.07.006
ID Item Name Supplier cat # Notes
2.3.1 Multichannel pipettes Pipet-Lite Multi Pipette L8–10XLS+; L8–20XLS+; L8–200XLS+ Rainin or equivalent
2.3.2 Water bath incubator Precision 260 Circulating Water Bath Thermo Fisher Scientific or equivalent
2.3.3 Thermomixer Eppendorf ThermoMixer Eppendorf or equivalent
2.3.4 Fluorometer Qubit Fluorometer ThermoFisher Scientific any version
2.3.5 Tubes Qubit Assay Tubes ThermoFisher Scientific Q32856
2.3.6 Sonicator Bioruptor Pico Diagenode B01060010 See Note 9.1 for alternatives
2.3.7 Tubes Bioruptor Pico 0.2 ml microtubes Diagenode C30010020
2.3.8 Thermocycler with heated lid T100 Thermal Cycler BioRad 1861096EDU or equivalent
2.3.9 PCR tubes MAXYMum Recovery™ PCR Tubes 0.2 ml Corning PCR-02D-L-C or equivalent
2.3.10 Low-bind 1.7 ml tubes DNA LoBind Tubes 1.5 ml Eppendorf 22431021 or equivalent
2.3.11 Minicentrifuge tubes Posi-Click tube, 1.7ml natural color Denville Scientific C2170 or equivalent
2.3.12 Mini centrifuge MyFuge™ 12 mini centrifuge Benchmark Scientific C1012 or equivalent
2.3.13 Benchtop Centrifuge Centrifuge 5425 Eppendorf 5405000042 or equivalent
2.3.14 Vacuum concentrator Vacufuge Concentrator 5301 Eppendorf 022820001 or equivalent. See Note 9.5 for alternative methods to concentrate the samples.
2.3.15 Magnetic rack for PCR tubes DynaMag™-96 Side Magnet
PCR Strip MagStand
ThermoFisher Scientific
Zymo Research
12331D
3DP-1002
or equivalent
or equivalent
2.3.16 Magnetic rack for 1.5 ml tubes DynaMag™-2 Magnet ThermoFisher Scientific 12321D or equivalent
2.3.17 Vortexer Fisherbrand™ Analog Vortex Mixer Fisher Scientific 02-215-414 or equivalent