Table 3.

Proteins whose levels are significantly altered by combination treatment with metformin and BMS-754807 compared with single agent treatment

Proteins	BMS-754807 + Metformin vs. BMS-754807		BMS- 754807 + Metformin vs. Metformin
	p value	Fold Change	p value	Fold Change
p27/KIP1	<0.0001	2.60	<0.0001	13.81
p-p27(T187)	<0.0001	2.27	<0.0001	5.11
KLF4	0.0010	1.71	<0.0001	2.32
c-Jun	0.0145	1.60	<0.0001	2.65
p-c-Jun(S63)	0.0001	1.53	<0.0001	2.73
Cleaved caspase-7	<0.0001	1.45	<0.0001	1.52
RRM2	<0.0001	−3.94	<0.0001	−6.05
Ki-67	<0.0001	−3.31	<0.0001	−8.22
Slug	<0.0001	−2.43	<0.0001	−3.51
Aurora A	<0.0001	−2.13	<0.0001	−4.56
p-Rb(S807/811)	<0.0001	−1.91	<0.0001	−4.73
CHAF1A	0.0122	−1.88	<0.0001	−9.76
p-Rb(S780)	<0.0001	−1.87	<0.0001	−4.16
HexokinaseII	<0.0001	−1.82	<0.0001	−1.84
BRCA1	<0.0001	−1.70	<0.0001	−3.28
Caveolin-1	0.0353	−1.65	<0.0001	−2.76
HIF-1a	0.0087	−1.49	0.0246	−1.49
LRP6	0.0101	−1.46	0.0002	−1.80
PIAS1	0.0211	−1.44	<0.0001	−2.81
p-Src(Y527)	0.0054	−1.43	<0.0001	−3.33
ATM	0.0002	−1.42	<0.0001	−2.06
p-EGFR(Y1068)	0.0117	−1.38	<0.0001	−1.98
p-SHC(Y317)	0.0032	−1.36	<0.0001	−1.70
Ezh2	0.0351	−1.32	<0.0001	−2.07
p-ALK(Y1604)	0.0370	−1.30	<0.0001	−2.06
p-FAK(Y576/577)	<0.0001	−1.29	0.0006	−1.24
STAT3	0.0008	−1.28	0.0012	−1.26
Notch1	0.0151	−1.26	<0.0001	−1.63
p-Beta-Catenin(S33/37/T41)	0.0069	−1.25	<0.0001	−1.49
ILK1	0.0015	−1.23	<0.0001	−1.42
STAT1	0.0042	−1.21	<0.0001	−1.43
c-Myc	0.0352	−1.21	<0.0001	−1.63
p-c-Myc(T58)	0.0396	−1.21	<0.0001	−2.21
Rb	0.0287	−1.17	<0.0001	−3.51
SCD1	0.0410	−1.17	0.0041	−1.26

The normalized data were log2 transformed for hypothesis testing. For each sample, the median value of three technical replicates was used for statistical analysis. Two-way ANOVA was used to test main effects of the different treatments and their interactions. p value < 0.05 was considered statistically significant.