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. Author manuscript; available in PMC: 2021 Jun 16.
Published in final edited form as: Nature. 2020 Dec 16;589(7842):462–467. doi: 10.1038/s41586-020-03052-3

Figure 4: TSS-proximal nucleosomes are asymmetric.

Figure 4:

(a) Black: Intrinsic cyclizability as a function of position along 601 DNA (supplementary note 18) Blue: abscissa value of the solid horizontal line (−0.196) is the mean intrinsic cyclizability along the 500 native +7 nucleosomal sequences represented in Library L (supplementary note 14). The height of the light blue background (0.011) is twice the s.e.m. (b) Schematic representing the design of the Cerevisiae Nucleosomal Library (supplementary note 4). The library contains DNA fragments taken from the 50 bp immediately to the left and right of the dyads of the ~10,000 nucleosomes in S. cerevisiae that have the highest NCP scores. (c) Mean intrinsic cyclizabilities of the 50 bp DNA fragments that lie immediately adjacent to the TSS proximal (red) or distal (blue) side of the dyads of various categories of nucleosomes (−9 through +9) (see supplementary note 17). Error bars are s.e.m. See supplementary Note 17 for the number of nucleosomes in each category over which data was averaged. (d) A subset of the data as in panel c, where the means were calculated considering only genes among the 10% most (left panel) or least (middle panel) expressed in S. cerevisiae. The right panel is identical to panel b, except for an altered y-axis scale. Error bars are s.e.m. See supplementary note 17.