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. Author manuscript; available in PMC: 2021 May 9.
Published in final edited form as: Nat Struct Mol Biol. 2020 Nov 9;28(1):50–61. doi: 10.1038/s41594-020-00522-0

Extended Data Fig. 8. ATP dependence of Mec1 activity.

Extended Data Fig. 8

(a) Standard Mec1 kinase assays without activator at 40 mM NaCl, or (b) with 200 nM Dna2(1-499) at 100 mM NaCl, were carried out at increasing concentrations of ATP. Activities are expressed as protein phosphates (Rad53 plus Dna2(1-499) when relevant) per Mec1 (monomer) per minute.

(c) Comparative ATPase (solid bars) and kinase (striped bars) activities of Mec1-Ddc2 and Mec1(F2244L)-Ddc2, in the presence or absence of Rad53 and Dpb11 (see Methods).

(d) Summary of phenotypes of all Mec1 mutants. The in vitro and in vivo phenotypes of the mutants are shown in the form of heat maps using Prism 8 GraphPad software.