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. Author manuscript; available in PMC: 2022 Jan 21.
Published in final edited form as: Cell Chem Biol. 2020 Nov 5;28(1):34–45.e6. doi: 10.1016/j.chembiol.2020.10.007
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Anti-His6-Tb Cisbio CAT# 61HISTLF
Anti-MBNL1 EMD Millipore Corporation CAT# MABE70
Anti-mouse IgG AlexaFluor488 Thermo Scientific Pierce CAT# 7074
Chemicals, Peptides, and Recombinant Proteins
Streptavidin XL-665 Cisbio CAT# 610SAXLF
MBNL1-His6 protein Expressed as described in (Chen et al., 2012) N/A
Critical Commercial Assays
qScript cDNA synthesis Quanta BioSciences CAT# 101414-100
Quick-RNA Miniprep Kit Zymo CAT# R1055
TRIzol Reagent Thermo Fisher Scientific CAT# 15596026
WST-1 Reagent Roche CAT# 5015944001
CellTiter-Glo 2.0 Promega CAT# G9242
SSIII reverse transcriptase Life Technologies CAT# 18080085
GoTaq DNA polymerase Promega CAT# M8295
SYBR Green Master Mix Life Technologies CAT# 4368708
Experimental Models: Cell Lines
DM1 Fibroblasts Coriell Institute GM03987
Healthy Fibroblasts Coriell Institute GM07492
DM1 conditional MyoD fibroblasts Arandel et al., 2017 N/A
Healthy conditional MyoD fibroblasts Arandel et al., 2017 N/A
F35T (FECD patient-derived corneal endothelial cells) Albert S. Jun, M.D., Ph.D.; Wilmer Eye Institute, Johns Hopkins Medical Institutions N/A
F20T (wild-type corneal endothelial cells) Albert S. Jun, M.D., Ph.D.; Wilmer Eye Institute, Johns Hopkins Medical Institutions N/A
Experimental Models: Organisms/Strains
Mouse: HSA LR20b Mankodi et al., 2000 N/A
Mouse: FVB/NJ The Jackson Laboratory 001800
Oligonucleotides
5’-Biotin-GCG(CUG)12-CGC Dharmacon N/A
Control siRNA (ON-TARGET plus) Dharmacon CAT# D-001810-04-05
siRNA-XRN1 (ON-TARGET plus) Dharmacon CAT# J-013754-11-0002
siRNA-DIS3 (ON-TARGET plus) Dharmacon CAT# J-015405-110002
siRNA-hRRP6 Dharmacon (Kammler et al., 2008) N/A
Primers used for RT-qPCR, see Table S1 Integrated DNA Technologies N/A
5’DY547-2’OMe-(CAG)6 Integrated DNA Technologies N/A
5’-Cy5-GCG(CUG)12-CGC Dharmacon N/A
5’-Cy5-GCG(CAG)12-CGC Dharmacon N/A
5’-Cy5-GCG(CUG)6(CAG)6-CGC Dharmacon N/A
5’-Cy5-r(G4C2)8 Dharmacon N/A
5’-Cy5-CGCGAATTCGCGTTTTCGCGAATTCGCG Integrated DNA Technologies N/A
Software and Algorithms
ImageJ NIH https://imagej.nih.gov/ij/download.html
Olympus Fluoview Software Version 3.0 Olympus http://www.olympuscanada.com/cpg_section/cpg_support_downloads.asp?id=1274
GraphPad Prism 7 GraphPad Prism Software, Inc. https://www.graphpad.com/scientific-software/prism/
Nucgen module of AMBER 16 AMBER 2016 https://ambermd.org/GetAmber.php