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. Author manuscript; available in PMC: 2021 Jun 14.
Published in final edited form as: Nat Struct Mol Biol. 2020 Dec 14;28(1):29–37. doi: 10.1038/s41594-020-00530-0

Extended Data Fig. 4 |. Single-particle cryo-EM maps docked into a subtomogram average of the axoneme.

Extended Data Fig. 4 |

a, Two views showing the single-particle cryo-EM maps of RS1, RS2, RS3S, N-DRC, and IDAc docked into the subtomogram average of the 96-nm repeat of the Chlamydomonas axoneme (EMD-6872). The subtomogram average is shown as a gray isosurface. b, Zoom-in view showing the map of RS3S. Density for RS3S is recovered in 25% of the particles following 3D classification (Extended Data Fig. 1b). RS3S interacts with two molecular staples of unknown identity. c, Zoom-in view showing the model and map for the N-DRC baseplate. Three N-DRC subunits (DRC1, DRC2, and DRC4) can be unambiguously identified. FAP91 interacts with all three.