Figure 7.

Splenic iNKT cells are intrinsically affected by loss of MyD88 signaling during acute MCMV infection. Frequency of IFN-γ⁺ donor (A) iNKT cells (CD45⁺TCRβ⁺CD1dtet⁺CD45.1⁻CD45.2⁺eYFP⁺) and (B) NK cells (CD45⁺TCRβ⁻NK1.1⁺CD45.1⁻CD45.2⁺) from the spleen and liver of B6.SJL recipients at 36 hours post-infection with MCMV. Donor cells were isolated from either MyD88 control (black) or MyD88 cKO (open) animals and enriched for iNKT cells prior to adoptive transfer. (C) Representative flow cytometry of IFN-γ production by indicated donor iNKT cells from the spleen and liver of B6.SJL recipients. Data are pooled from or representative of two independent experiments (n=6–7) and error bars indicate SEM.