Figure 3:

DTH responses to Leishmania challenge are intact after treatment with tofacitinib. (a) Naive or immune mice (infected for >10 weeks) were challenged with Leishmania at the contralateral ear and mice were treated with tofacitinib or vehicle. (b) DTH reaction was measured at 72 hours post-challenge. (c and d) Cells were stimulated with PMA and ionomycin in the presence of brefeldin A for 4 hours and analyzed by flow cytometry for the expression of intracellular IFN-γ. (c) Representative contour plots and (d) bar graphs are representative of 2 individual experiments with 4–5 mice per group with similar results. Gating strategy: live, singlets, CD3, CD4. **p ≤ 0.01 and ***p ≤ 0.001