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. Author manuscript; available in PMC: 2022 Jan 26.
Published in final edited form as: Biomater Sci. 2021 Jan 26;9(2):471–481. doi: 10.1039/d0bm00518e

Fig. 3.

Fig. 3

Quantitation of PSA bound on IONP-Neu particles. (a) SDS-PAGE image of free PSA samples collected from the 100 kDa spin filter flow through of PSA control, IONP-Neu-PSA and IONP-Neu. (b) BCA protein assay with PSA standard curve ranging from 0–1000 mg/mL. The absorbances of the spin filter flow through of PSA control, IONP-Neu-PSA and IONP-Neu were measured and fitted to the PSA standard curve for free PSA concentration calculation. The inset is the enlarged linear portion of the curve at low concentrations. (c) Statistical analysis of the PSA concentration data from SDS-PAGE image and BCA protein assay. The concentration of PSA controls from SDS-PAGE and BCA were both normalized to 100%. P value < 0.05 is denoted as *, < 0.005 as ** and < 0.0005 as ***.