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In vitro apoptotic cell labeling characterized by confocal microscopy. 4T1 cells were either treated with 20 μM PTX for 24 hours to induced apoptosis or received no PTX treatment as control. Both the treated and untreated cells were labeled with PI (red), and either Annexin V-AF488 (green) or IO NP-Neu-PSA-Cy5 (green). All the stained 4T1 cells were then mounted onto glass slides with anti-fade agent.
