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. Author manuscript; available in PMC: 2021 May 23.
Published in final edited form as: Nat Mater. 2020 Nov 23;20(2):260–271. doi: 10.1038/s41563-020-00853-9

Extended Data Figure 2. Patterning of kidney structures in bioprinted organoids generated from varying starting cell numbers.

Extended Data Figure 2.

A. Immunofluorescence of organoids from a single starting differentiation used to generate manual organoids (5 × 105 cells) versus bioprinted organoids generated from as few as 4,000 cells. Representative images from n = 3 organoids stained. Scale bars represent 200μm. B. Differentiation timecourse of bioprinted organoids generated using the MAFBmTagBFP2 reporter iPSC line. C. MAFBmTagBFP2 bioprinted organoids on the same Transwell filter with 4K, 50K or 100K of cells per organoid showing fluorescence reporter imaging (blue) and staining for differentiation (ECAD,green; LTL, blue; GATA3, red; NPHS1, purple). Images are representative of at least n = 3 organoids. Scale bars represent 200μm. D. MAFBmTagBFP2 bioprinted organoids on the same Transwell filter all generated using 100K of cells per organoid showing live fluorescence imaging (blue) and staining for differentiation (ECAD,green; LTL, blue; GATA3, red; NPHS1, purple). Scale bars represent 200μm. Representative wells from at least n = 3 are shown, with representative stained organoids alongside.