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. Author manuscript; available in PMC: 2021 Feb 3.
Published in final edited form as: J Am Chem Soc. 2020 Dec 30;143(2):805–816. doi: 10.1021/jacs.0c09559

Figure 2.

Figure 2.

Exonuclease-based fluorescence profiling of ATPwt binding to various targets. (A) Time-course plot of ATPwt digestion by Exo III and Exo I in the absence and presence of various ribonucleotides at a concentration of 250 μM. (B) The t1/2 ratio was used to determine the relative binding affinity to each ligand at 100 and 250 μM. The red line indicates a t1/2 ratio of 1, which reflects no inhibition of aptamer digestion.