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. Author manuscript; available in PMC: 2021 Feb 3.
Published in final edited form as: J Am Chem Soc. 2020 Dec 30;143(2):805–816. doi: 10.1021/jacs.0c09559

Figure 6.

Figure 6.

Exonuclease-based fluorescence profiling of thrombin-binding aptamers. Time-course plot of digestion of 500 nM (A) Tasset, (B) Bock, and (C) Bock-hang by Exo III and Exo I in the absence (black) and presence of 500 nM human α-thrombin (red) or human factor X (brown). (D) The half-life (t1/2) ratio of the digestion reaction was used to determine relative aptamer binding affinity to α-thrombin and factor X. The y-axis is log2-scaled. The red line indicates a t1/2 ratio of 1, which means there was no inhibition of aptamer digestion.