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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Oncogene. 2020 Nov 16;40(3):592–602. doi: 10.1038/s41388-020-01553-z

Figure 2.

Figure 2.

Expression of proteins responsible for fatty acid synthesis is increased in prostatic intraepithelial neoplasia (PIN) and adenocarcinoma (ADC) by prostate-specific transgenic expression of Myc in Hi-Myc mice. Representative immunohistochemical images for (a) ATP citrate lyase (ACLY), (b) acetyl-CoA carboxylase 1 (ACC1), and (c) fatty acid synthase (FASN) in normal prostate, PIN, and ADC of wild-type (WT) or Hi-Myc mice (40× oil objective magnification; scale bar- 50 μm). Sections were stained with a stromal marker α-smooth muscle actin (SMA) protein. Nuclei were visualized by staining with 4′,6-diamidino-2-phenylindole (DAPI), which is a dye that strongly binds to adenine-thymine rich regions in DNA. d Bar graphs showing quantitation of ACLY, ACC1, and FASN protein expression by ImageJ software. Results shown are mean ± SD (n = 6). Statistical significance was determined by Student’s t-test.