Figure 1.

Sufficiency of a single dose of tamoxifen (Tmx) in activating the nuclear translocation of Cre recombinase that triggered DNA recombination to drive tdT expression in NC and NC-derived tissues. A-B: Low (A) and high (B) -power images of transverse sections of the cranial region of a vehicle (Veh)-treated Sox10-iCreER^T2 mouse embryo at E8.5 (12-somite). Immunosignals of Cre (green) in the cytoplasm (arrowheads in B) and Sox10 (magenta) in the nuclei were visualized. White dashed lines in A outline the foregut diverticulum with non-specific staining (40). TN: trigeminal NC tissue; BA1: branchial arch 1; OE: optic eminence. C: High-magnification images of the BA1 region in a transverse section of an E8.5 (9-somite) Sox10-iCreER^T2 mouse embryo with tamoxifen activation of Cre (Tmx^E7.5). Arrows point to the Cre immunosignals (green) within nuclei. D: Images of whole mount (D₁) and sagittal section (D₂) of the tongue from a E12.5 Sox10-iCreER^T2/tdT mouse embryo with tamoxifen activation of Cre (Tmx^E7.5). Tongue epithelium was immunoreacted with antibody against E-cadherin (green) in D₂. E: Bright field (E₁) and tdT fluorescent (E₂) images of mouse dorsal root ganglia (arrows) of a Sox10-iCreER^T2/tdT mouse at 8 wk with tamoxifen activation of Cre (Tmx^E7.5). Arrows point to the dorsal root ganglia. Scale bars: 50 μm in A and D (single-plane laser scanning confocal); 10 μm in B and C (single-plane laser scanning confocal); 1 mm in E (stereomicroscopy).