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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: J Immunol Methods. 2020 Dec 14;489:112943. doi: 10.1016/j.jim.2020.112943

Figure 2:

Figure 2:

Digitonin wash reduced popping without impacting lymph node slice viability. (a) The percentage of vaccinated LNs that popped during slicing. Each dot represents one experiment. Total n for nodes is shown above each bar. * indicates p=0.0470 by Fisher Exact Test. (b) Representative plot of flow cytometric analysis of viability. Live cells were defined as Calcein AM positive and 7-AAD negative. Data from an unwashed LN from a naïve female mouse are shown. (c,d) Overnight viability of slices from male and female mice that were vaccinated (c) or naïve (d). Each dot represents one slice. Bars show mean and standard deviation. ns indicates p > 0.05 by unpaired t test.