Figure. 1.

Gemcitabine treatment of PDAC cells increases Cav-1 expression and albumin uptake in vitro. A-D. AsPC-1 and HPAF-II cells were treated with gemcitabine (50 nM) for 8, 24, 48 hours and Cav-1 expression was measured by qRT-PCR (A,B) and western blot (C,D). Band quantitation with Image J software shown underneath (C,D). E-F. AsPC-1 and HPAF-II cells were treated with increasing doses of gemcitabine (25–100 nM for AsPC-1 and 5–20 nM for HPAF-II) for 24 hours or 48 hours followed by measurement of intracellular human albumin expression by western blot. G. Western blot of AsPC-1 and HPAF-II cells treated with gemcitabine for 7 days at increasing gemcitabine concentrations compared to control (Ctrl) lysates made prior to treatment with 7 days of gemcitabine. *p < 0.05, **p < 0.01, ***p < 0.001, ****p<0.0001.