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. Author manuscript; available in PMC: 2021 May 1.
Published in final edited form as: J Biomed Mater Res A. 2020 Aug 14;109(5):733–744. doi: 10.1002/jbm.a.37058

FIGURE 6.

FIGURE 6

Effects of transforming growth factor-beta 1 (TGF-β1) in a scaffold coculture of smooth muscle cells (SMCs) and fibroblasts. WKY3M22 SMCs (top panels) and NIH 3T3 fibroblasts (bottom panels) were grown to confluence on the aligned and unaligned sides of the scaffold, respectively. TGF-β1 was then added to the medium and the coculture was incubated for 3 days. Cells were fixed and stained with R457 antiserum for FN (green) and with anti-alpha smooth muscle actin (α-SMA) antibody (red). The fold changes in mean fluorescence intensity of SMCs compared to fibroblasts are shown in white (average of 6–8 fields of view). Scale bar = 50 μm