Figure 5.

Impact of HIV-1 Tat and cocaine on redox-related protein expression and energy sensor protein in vivo. HIV-1-inducible Tat transgenic (iTat) or saline-administered (control) mice received daily intraperitoneal injections of saline or doxycycline (Dox; 100 mg/kg/d) over 14 days, with or without s.c. injection of cocaine (10 mg/kg/d). Following treatment, equal amounts of protein lysate from harvested brain tissue were resolved, and protein expression was analyzed by Western blot: GSS (A), SOD (C), CAT (E), GFAP (G), and AMPK (I). Figure B, D, F H, and J represent the percent densitometric value protein levels (% control). Data are expressed as the mean ± SE of three independent experiments.