Table 3. Comparison of enhancement of RNAi efficiency in ECB tissue culture for multiple target genes due to nuclease inhibitor Zn2+.
The relative expression (in fold change plus and minus standard error) of each target gene after incubation of excised midguts in media containing 100 ng/ml of dsGFP or dsRNA specific to each target gene are presented.
| RNAi Efficiency in ECB Midgut Cultures | ||||||
|---|---|---|---|---|---|---|
| Without Zn2+ | With Zn2+ | |||||
| Target gene | dsGFP | dsTarget | Significant suppression | dsGFP | dsTarget | Significant suppression |
| OnLgl | 1.00±0.06 | 0.83±0.20 | No | 1.01±0.10 | 0.95±0.21 | No |
| OnArf72A | 1.08±0.23 | 0.78±0.17 | No | 1.14±0.33 | 0.08±0.03 | 92% |
| OnChc | 1.03±0.13 | 1.42±0.10 | No | 1.04±0.16 | 0.69±0.07 | 33% |
| OnAP50 | 1.02±0.11 | 1.19±0.29 | No | 1.14±0.33 | 0.08±0.03 | No |
| OnVha16 | 1.04±0.16 | 0.38±0.04 | 64% | 1.06±0.20 | 0.44±0.14 | 59% |
| OnVhaSFD | 1.10±0.21 | 0.68±0.10 | 38% | 1.04±0.16 | 0.69±0.07 | 33% * |
*
Italicized averages are trends (not statistically significant). Abbreviations: On, Ostrinia nubilalis; Lgl, lethal giant larvae; Chc, clathrin heavy chain; AP50, clathrin coat adaptor protein AP50; Arf72A, ADP ribosylation factor 72A; Vha16, V-type proton ATPase 16 kD proteolipid subunit; VhaSFD, V-type proton ATPase subunit H.