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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Acta Biomater. 2020 Nov 21;121:431–443. doi: 10.1016/j.actbio.2020.11.025

Figure 4:

Figure 4:

Five-stage elastic fiber discontinuity scale using longitudinal sections of the artery. The scale ranges from 1 (continuous fibers) to 5 (discontinuous fibers). Several examples are provided for each stage to illustrate variability. Elastic fibers are in the external elastic lamina (EEL) at the border of media and adventitia. Verhoeff-Van Gieson (VVG) stain: elastin is black, collagen is red, smooth muscle is brown. The scale bars at the bottom left corner of each section represent 100μm.