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. 2021 Jan 20;13:596039. doi: 10.3389/fnmol.2020.596039

Figure 2.

Figure 2

Administration of 70 mg/kg Ax enhances GFAP+ cells proliferation in SVZ and increases the portion of NSCs in penumbra after dMCAO. (A) Representative immunostaining images showing colocalization of BrdU (red) with DCX (green) or GFAP (green) in SVZ. Scale bar: 20 μm. (B) Quantitative analysis of the average number of BrdU+ and DCX+ cells in SVZ in group dMCAO + Vehicle and dMCAO + Ax-70 mg/kg. (C) Quantification of the average number of colocalization of BrdU (red) and GFAP (green) in SVZ. **P < 0.01 vs. dMCAO + Vehicle group. (D) Typical immunostaining images showing colocalization of Nestin (red) with GFAP (green) in penumbra. Scale bar: 20 μm. (E) Quantitative analysis of the percentage of Nestin+ and GFAP+/GFAP+ in penumbra in group dMCAO + Vehicle and dMCAO + Ax-70 mg/kg. ***P < 0.001 vs. dMCAO + Vehicle group. Ax, ambroxol; dMCAO, distal middle cerebral artery occlusion; GFAP, glial fibrillary acidic protein; CC, corpus callosum; Str, striatum; LV, lateral ventricle.