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. Author manuscript; available in PMC: 2021 May 30.
Published in final edited form as: Nat Chem Biol. 2020 Nov 30;17(2):213–221. doi: 10.1038/s41589-020-00656-8

Figure 5. LCMS characterization of metabolites produced by WT and mutant S. cacaoi and S. tendae.

Figure 5.

a. The transformation of 5’-OAP into AHOAP. b. HPAEC chromatograms at 260 nm of nucleotide fraction of culture media of S. tendae ΔnikL::kanR and ΔnikK::kanR, and S. cacaoi ΔpolQ2. The identities of 5’-OAP, 2’-OAP, and KOAP were determined by isolation and characterization with NMR. OABP was identified by co-injection with an authentic standard. c-e. LC-HRMS analysis of S. tendae wt, ΔnikK::kanR, and ΔnikL::kanR and S. cacaoi ΔpolQ2 culture media. Shown are EIC for 2’-OAP and 5’-OAP (m/z 395.0486 ± 0.0020; c), OABP (m/z 496.9969 ± 0.0025, d), and HKOAP (m/z 427.0355 ± 0.0021, e). The observations were reproducible for 2-3 different clones for each mutant strain. The culture was repeated twice for each clone.