Extended Data Fig.1 |. Characterization of Hist1H1 allele mutations and deletions in DLBCL.

a, A PanCancer Atlas cancer mutation survey was performed using the cBioPortal to search the cumulative mutation frequency of HIST1H1A, HIST1H1B, HIST1H1C, HIST1H1D, HIST1H1E across a total of 10,953 non-redundant patient samples across all cancer types. b, HIST1H1B-E mutation landscape across non-redundant TCGA and BCCA DLBCL samples (number of samples indicated for each H1 isoform); location of mutations is plotted onto protein structure, with bar height corresponding to mutation counts, total frequency of mutations in each H1 isoform is plotted to the right (red bars). c, Prevalence of H1 mutant vs H1 wild-type cases in ABC- and GCB-DLBCL cases (Fisher’s exact test for enrichment in ABC or GCB-DLBCL, P>0.05) d,Prevalence of H1A-E missense mutations and heterozygous loss in MCD subtype DLBCL and their enrichment (-log10 Pvalue) over non-MCD DLBCLs. e, Summary table of frequency of mutations and heterozygous loss of HIST1H1A, HIST1H1B, HIST1H1C, HIST1H1D, HIST1H1E in DLBCL subtypes (MCD, ST2, BN2, EZB, N1, AP53). f, Co-occurrence as odds ratio (OR) and −log10 p-value among HIST1H1 alleles in 101 germline-matched WGS DLBCLs. g, Frequency and location of missense mutations for HIST1H1A, HIST1H1B, HIST1H1C, HIST1H1D, HIST1H1E in 101 germline-controlled DLBCLs. h, Quantile-quantile plot showing the P-values for SNV across 101 germline-matched WGS DLBCLs. Driver analysis derived as probability of mutation count greater than or equal to the observation mutation count under Gamma-Poisson distribution expected P-values for SNVs. Shaded in gray zone contains mutant genes with FDR<0.01, depicted as blue or red dots, several of which are listed in the figure. i, Oncoprint for HIST1H1A, HIST1H1B, HIST1H1C, HIST1H1D, HIST1H1E in 101 germline-matched WGS DLBCLs. j, Co-occurrence as odds ratio (OR) and −log10 p-value among HIST1H1alleles in 101 germline-matched WGS DLBCLs