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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Bone. 2020 Dec 16;144:115819. doi: 10.1016/j.bone.2020.115819

Figure 3: GATA4 regulates the number of mesenchymal stem cells.

Figure 3:

(A-C) Bone marrow cells from WT and Gata4 Prx-cKO mice were cultured in MesenCult media. (A) Gata4 mRNA expression levels in undifferentiated cells from WT and Gata4 Prx-cKO mice. (B) Cells were stained with Giemsa and (C) the number of Giemsa colonies in multiple wells was quantified using ImageJ. (D and E) MSCs were analyzed for the expression of Sca1+/CD45−/CD34−/CD44 markers using flow cytometry. (F) The number of Sca1+/CD45−/CD34−/CD44lo (MPC1) cells and Sca1+/CD45−/CD34−/CD44hi (MPC2) from D and E are graphed. (G) Nestin expression was measured by qPCR from cells grown as in parts A-C. (H) Immunofluorescence of nestin in WT and Gata4 Prx-cKO mice (I) Percentage of nestin positive cells in three replicates as in part H. Student’s t test; * P < 0.05; *** P < 0.001 compared with wild-type.