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. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Bone. 2020 Dec 16;144:115819. doi: 10.1016/j.bone.2020.115819

Figure 6: WNT10B regulates GATA4 expression.

Figure 6:

(A) Bone marrow cells from WT and WNT10BKO mice were cultured in MesenCult media. Cells were stained with Giemsa and (B) the number of Giemsa colonies (CFU) was quantified using ImageJ. (C) MSCs were analyzed for the expression of Sca1+/CD45−/CD34−/CD44+ markers using flow cytometry. (D) Bone marrow cells from WT and WNT10BKO mice were cultured in MesenCult media. Gata4 mRNA expression levels were analyzed by qPCR in undifferentiated cells from WT and WNT10BKO mice. (E) Bone marrow cells from WT and WNT10BKO mice were cultured in MesenCult media. Cells were fixed and stained with an antibody to GATA4 (pink). Nuclei were identified with DAPI (blue). (F) WT osteoblasts were untreated or treated with 100 ng/mL rWNT10B for 24 hours and then lysed for RNA. GATA4 mRNA was detected by qPCR.