Other enzyme-responsive multimodal imaging. ALP-specific activation of P-CyFF-Gd resulted in enhanced MR and FL contrast in a) xenograph and b,c) orthotopic tumor models. Imaging in the orthotopic tumor model enabled image-guided surgery to ressect tumor tissue. In (b) and (c), I represents the activatable probe (P-CyFF-Gd), II represents the probe without the ALP substrate (P-Cy-Gd), and III represents the probe and an ALP activity inhibitor (P-CyFF-Gd + Na3VO4). Reproduced with permission.[89] Copyright 2019, American Chemical Society.