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. Author manuscript; available in PMC: 2021 Jul 4.
Published in final edited form as: Nat Struct Mol Biol. 2021 Jan 4;28(1):92–102. doi: 10.1038/s41594-020-00534-w

Extended Data Fig. 2: 2D class averages of nsEM images with different versions of the core complex.

Extended Data Fig. 2:

Core complexes without MBP or with MBP fused at the N-terminus of Rec102, Spo11 or Rec104 are shown. A cartoon of the presumed arrangement of the subunits and the position of the MBP electron density is shown. With the MBP-tagged Spo11 construct, the electron density of MBP is located at a similar position to the Rec102- or Rec104-tagged constructs. This is consistent with the observation that the N-terminus of Spo11 frequently crosslinks with Rec104 (pink lines in Fig. 2a), suggesting that the N-terminus of Spo11, absent from the structural model, is flexible and perhaps directly contacts Rec102/Rec104. The observation of a single MBP density for all three subunits tested provides further support for the 1:1:1:1 stoichiometry of the core complex. Complexes with MBP-tagged Ski8 were not well behaved and could not be purified.