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. Author manuscript; available in PMC: 2021 Jul 4.
Published in final edited form as: Nat Struct Mol Biol. 2021 Jan 4;28(1):92–102. doi: 10.1038/s41594-020-00534-w

Fig. 6: Mutations that affect Spo11 DNA binding compromise DSB formation.

Fig. 6:

a. Binding of wild-type or mutant Spo11-containing core complexes to a 25-bp hairpin substrate with 5′-TA overhang (DSB) or a DNA mini-circle. The affinity of R344A was decreased 3-fold for DNA duplex and 4-fold for DNA ends. For K178A, the affinity was decreased 27-fold for the ends and could not be measured on duplex DNA because binding did not reach saturation.

b. Labeling of Spo11-oligo complexes. Representative gels of n = 2 assays are shown above; quantification is plotted below (mean and range from two experiments).

Uncropped blot and gel images and data for graphs are provided in the Source Data.