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. Author manuscript; available in PMC: 2021 Apr 2.
Published in final edited form as: Prostate Cancer Prostatic Dis. 2020 Jul 21;24(1):268–277. doi: 10.1038/s41391-020-0246-y

Figure 3. BRD4 mediates SNAI1 and SNAI2 expression through promoter interactions.

Figure 3.

A, Immunoblot of phospho-Smad3 (pSmad3) and Smad3 in DU145 cells treated with either 0.01% DMSO or 100 nM MZ1 for 21 hours, serum starved for 2 hours under the same conditions and then dosed with 5 ng/mL TGFß for the indicated time. The ratio of pSmad3:Smad3 illustrates Smad3 activation and quantifications are relative to control. Blots shown are representative of two independent experiments.

B, DU 145 cells treated with either 400 nM of (–)JQ1 or (+)JQ1 for 24 hours and then harvested for ChIP. BRD4 interacts with the SNAI1 and SNAI2 promoters and is displaced upon exposure to (+)JQ1. Results from two independent experiments are shown. Statistical analyses were performed using the Student’s t test. Significant differences: ns, nonsignificant, P > 0.05; *.

C, Publicly available ChIP-seq datasets performed on DHT or DHT + JQ1-treated VCaP cells were analyzed for the binding of BRD4 and RNA Pol II at the SNAI1 and SNAI2 locus. The analyzed datasets are accessible on the GEO platform (GSE55062 (16)). Data visualization was performed using Integrative Genomics Viewer (IGV).