Table 1.

Primer sets used in the site-directed mutagenesis of the cDNA encoding human SULT1E1 allozymes

SULT1E1 Allozyme and Corresponding Amino Acid Substitution	Mutagenic Primer Set	Minor Allele Frequency
SULT1E1-A43D	5’-GATGATCTTGTCATTGACACCTACCCTAAATCTGGT-3’ 5’-ACCAGATTTAGGGTAGGTGTCAATGACAAGATCATC-3’
SULT1E1-A131P	5’-TATCTTTGCCGGAATCCAAAGGATGTGGCTGTTTCC-3’ 5’-GGAAACAGCCACATCCTTTGGATTCCGGCAAAGATA-3’	(0.00007–0.0002)
SULT1E1-R186L	5’-AAGGGAAAGAGTCCACTTGTACTATTTCTTTTCTAC-3’ 5’-GTAGAAAAGAAATAGTACAAGTGGACTCTTTCCCTT-3’	(0.000008–0.00002)
SULT1E1-P214T	5’-TTCCTGGAAAGGAAGACATCAGAGGAGCTTGTGGAC-3’ 5’-GTCCACAAGCTCCTCTGATGTCTTCCTTTCCAGGAA-3’	(0.000008–0.00008)
SULT1E1-D220V	5’-TCAGAGGAGCTTGTGGTCAGGATTATACATCATACT-3’ 5’-AGTATGATGTATAATCCTGACCACAAGCTCCTCTGA-3’	(0.000008)