Table 4.
Kinetic constants of the human SULT1E1 allozymes in catalyzing the sulfation of diethylstilbestrol
| SULT1E1 allozyme | Vmax (nmol/min/mg) | Km (μM) | Vmax/Km (ml/min/mg) |
|---|---|---|---|
| SULT1E1-WT1 | 8.54 ± 0.30 | 2.27 ± 0.19 | 3.77 ± 0.18 |
| SULT1E1-A43D | 6.10 ± 0.12**** | 1.00 ± 0.08**** | 4.70 ± 0.19*** |
| SULT1E1-A131P | 9.32 ± 0.65**** | 3.17± 0.23**** | 2.94 ± 0.01*** |
| SULT1E1-R186L | 11.18 ± 0.38**** | 4.07± 0.36**** | 2.76 ± 0.15*** |
| SULT1E1-P214T | 1.56 ± 0.04**** | 2.32 ± 0.15 | 0.68 ± 0.03*** |
| SULT1E1-D220V | 3.56 ± 0.16**** | 1.53 ± 0.24**** | 2.35 ± 0.27*** |
Data shown represent mean ± SD derived from 3 independent experiments. One-way ANOVA was performed followed by Tukey’s post hoc analysis.
****
Statistical significant p<0.0001 from SULT1E1-WT.
1
Wild-type human SULT1E1