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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Stem Cells. 2020 Dec 23;39(2):170–182. doi: 10.1002/stem.3296

Figure 3. ASC-S promotes iCM recovery post-UW treatment.

Figure 3.

(A) Dynamics of beating rate recovery in iCM after exposure to UW at 4°C for 4 hours, followed by incubation in RPMI media alone or supplemented with ASC-S at 37°C for 24 hours. Time “0” represents the moment when UW solution was exchanged to RPMI ± supplements. (B, C) Prevalence of cleaved caspase-3+ iCM (B) and beating velocity of iCM (C) evaluated after cells were exposed to UW for 4 hours, followed by incubation in RPMI ± ASC-S for 24 hours. (D) Dynamics of beating rate recovery in iCM treated with ASC-BM or ASC-S either only during UW incubation, only during the recovery phase, or during both incubations. (E) Beating velocity of iCM evaluated after cells were exposed to UW/supplements (ASC-BM or ASC-S) at 4°C for 4 hours, followed by incubation in RPMI ± supplements (ASC-BM or ASC-S) for 24 hours. Statistics: (A-E): one-way ANOVA with Tukey’s post hoc test was performed: n=6 for each graph; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.