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. Author manuscript; available in PMC: 2021 Feb 3.
Published in final edited form as: DNA Repair (Amst). 2020 Nov 25;97:103026. doi: 10.1016/j.dnarep.2020.103026

Fig. 2. DPC-containing plasmids are repaired via HR in purified mitochondria.

Fig. 2.

A. Schematic representation of mitochondrial DPC repair. Plasmid pM13 Mito (depicted by green oval with blue arc) harboring a DPC (indicated in red) within the portion of pM13 Mito that is homologous to the mitochondrial genome (depicted in blue) is electroporated into purified mitochondria (indicated by blue oval), recovered, and subjected to SSPE-qPCR using a pair of primers (indicated by Blue and Green arrows) that, respectively, hybridize to mitochondrial DNA- and M13-specific portions of the plasmid. B. Plasmid pM13 Mito containing a single DPC residue was electroporated into mitochondria purified from human HEK293T cells, recovered following a two- or three-hincubation period, and analyzed by SSPE-qPCR as depicted in (A). Results depict mean percent repair +/− the SEM, N = 2 and 7 (two-hour and three-hour incubation, respectively).