Figure 3.

Effects on the infarct volume and functional recovery of delayed (6 h post pMCAO) PACAP daily administration, 2 and 8 days after brain ischemia. Male C57BL6 mice were treated with a saline solution (control, sham) or with different concentrations of PACAP. (A) Illustration of the brain infarct zone after NaCl (control) or PACAP 1 µg/µl intranasal administration, 8 days after pMCAO. (B) Cerebral ischemic infarct volume (mm³) 8 days after pMCAO. (C, D) Beam test indicating cross times and number of slips of mice (sham and stroked animals) on 9 mm beam 2 and 8 days after stroke. (E, F) Adhesive removal test with the time taken for first contact and adhesive removal from mice paws 2 and 8 days after stroke. PACAP treatment promoted functional recovery and reduced cerebral ischemic lesions in stroke mice. Errors are reported as Mean ± S.E.M and n ≥ 8 animals per group. (*P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001).