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. Author manuscript; available in PMC: 2022 Jan 18.
Published in final edited form as: Angew Chem Int Ed Engl. 2020 Nov 18;60(3):1220–1226. doi: 10.1002/anie.202008625

Figure 2. Competitive binding experiments of 5 and (+)-JQ1 by fluorescence anisotropy and a commercial AlphaScreen assay demonstrates selectivity for BRD4 D1.

Figure 2.

(A) Competitive binding curves of 5 (in red) and (+)-JQ1 (in black) by fluorescence anisotropy against BRD2 D1, BRD4 D1, and BRD4 D2. (B) IC50 values of 5 and (+)-JQ1 by fluorescence anisotropy and a commercial AlphaScreen assay.

a Data represents the mean and SEM of biological replicates.

b IC50 values were determined by a commercial AlphaScreen assay. Data represents the mean of two biological replicates.

c 61% inhibition at 100 μM.