Representative western blots and densitometry analysis of Tyr-972 phosphorylated insulin receptor in (A) skeletal muscle, (B) adipose tissue, and (C) liver of Cyp4a12tg mice post 15 week of diet feeding. Quantitative protein expression is normalized to total insulin receptor as a loading control (n=4-6 per group); CD, Control diet; HFD, High fat diet; DOX, Doxycycline; SOLA, 20-HETE antagonist; *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001 using one-way ANOVA with post-hoc Tukey’s test.