Representative western blots and densitometry analysis of Ser-307 phosphorylated and total insulin receptor substrate-1 in (A) skeletal muscle, (B) adipose tissue and (C) liver of Cyp4a12tg mice. Quantitative protein expression is normalized to IRS-1 (for p-IRS1-ser307) and to β-actin/β-tubulin (for IRS1) as loading control (n=4-6 per group); CD, Control diet; HFD, High fat diet; DOX, Doxycycline; SOLA, 20-HETE antagonist; *p<0.05, **P<0.01, ***p<0.001, using one-way ANOVA with post-hoc Tukey’s test.