Figure 1.
MMA+3 and UA produce opposing effects on PARP-1 activity in mouse thymus cells. Mouse thymus cells were exposed in vitro to MMA+3or UA for 18 h and PARP-1 activity was measured using the mouse PARP/Apoptosis ELISA kit from R&D Systems. (A) Inhibition of PARP-1 activity caused by exposure to 0, 50, 100, and 500 nM MMA+3. (B) Increased PARP-1 activity produced by exposure to 0, 0.5, 5, and 50 μM UA UA. Data are presented as mean ± SD. *Statistically significant difference (p<0.05) in one-way ANOVA followed by a Dunnett’s t-test compared to the control group.