Table 2.
Benchmarking of the PEdELISA microarray platform to commercial immunoassay platforms in cytokine detection.
| Diagnostic System | Cost/96 Assays | Instrument Cost | *Assay Time | LOD (pg/mL) | Sample Volume (μL) | Plexity | Ref |
|---|---|---|---|---|---|---|---|
| Colorimetric ELISA | $100–500 | $5,000 | > 4 hrs | 1–10 | 100 | 1 | (rndsystems.com) |
| Luminex | $1000–8000 | >$50,000 | > 4 hrs | 0.1–100 | 25–50 | 2–65 | (thermofisher.com) |
| Quanterix SIMOA | >$1500 | >$ 100,000 | ~2.5 hrs/96 plate | 0.002–1 | 100 | 1–6 | (quanterix.com) |
| Bio-Techne Ella | $2000–4500 | >$60,000 | ~1.5 hrs/16 samples | 0.2–2 | 25 | 1–8 | (proteinsimple.com/ella.html) |
| PEdELISA microarray | <$50 | <$5,000 | ~30 min/8 samples | 0.2–3 | 15 | 1–8 |
*
Assay Time refers to the sample-to-answer time, i.e., the total time between sample loading and delivery of analyzed data. Note that the assay and instrument cost of the commercial systems were based on their market prices and may not reflect the production cost. All the information was estimated in a range to the best of our knowledge based on the platforms’ website, online manual/brochure, quote, and do not count for marketing strategies, e.g. discount.