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. Author manuscript; available in PMC: 2022 Feb 15.
Published in final edited form as: Toxicology. 2020 Dec 24;449:152662. doi: 10.1016/j.tox.2020.152662

Figure 3. Hepatic bile canaliculi are better retained over time in physiologic medium.

Figure 3.

MPCCs were cultured for 4 weeks in (a) physiologic or (b) traditional culture medium and then assessed for bile canaliculi functionality, with the CDCFDA transporter dye, and corresponding phase contrast imaging. Scale bar represents 250 μm. (c) The number of bile canaliculi branches per PHH island (n=5) in MPCCs cultured for 4 weeks in the two media types. (d) The relative area of excreted CDF per PHH island (n=5) in MPCCs cultured for 4 weeks in the two media types. Error bars represent SD. *,** represent p<0.05 and p<0.01, respectively.