Skip to main content
. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Hypertension. 2021 Jan 4;77(2):582–593. doi: 10.1161/HYPERTENSIONAHA.120.16589

Figure 5. ClC-6 electrophysiological properties.

Figure 5.

(A) Representative traces showing ClC-6 activity at different holding potentials, which were used to determine the single-channel current-voltage relationship. (B) Western blot of purified ClC-6 protein used for electrophysiological studies. Prediected molecular weight is 97 kDa plus a 28 kDa GST-tag. (C) Current-voltage (I-V) plot of voltage dependent ClC-6 conductance in bilayers composed of 80 mol% DPhPC and 20 mol% DPhPS in a 350:150 mM KCl, pH 7.4 bath solution gradient (n=15). Reversal potential is about −8 mV, which corresponds to non ideal anion selectivity. Error bars are ± SEM.