Table 1:
Summary of quantitative real-time RT-PCR oligonucleotide primers used in measuring mRNA expression in mitochondrial dynamics, mitochondrial biogenesis, and housekeeping genes in Control SWR/J, diabetic TH and SS31 treated TH mice.
Gene | DNA sequence (5′−3′) | Product size |
---|---|---|
Mitochondrial dynamics genes | ||
Drp1 | Forward primer ATGCCAGCAAGTCCACAGAA | 86 |
Reverse primer TGTTCTCGGGCAGACAGTTT | ||
Fis1 | Forward primer CAAAGAGGAACAGCGGGACT | 95 |
Reverse primer ACAGCCCTCGCACATACTTT | ||
MFN1 | Forward primer GCAGACAGCACATGGAGAGA | 83 |
Reverse primer GATCCGATTCCGAGCTTCCG | ||
MFN2 | Forward primer TGCACCGCCATATAGAGGAAG | 78 |
Reverse primer TCTGCAGTGAACTGGCAATG | ||
Opa1 | Forward primer ACCTTGCCAGTTTAGCTCCC | 82 |
Reverse primer TTGGGACCTGCAGTGAAGAA | ||
Housekeeping genes | ||
β-Actin | Forward primer AGAAGCTGTGCTATGTTGCTCTA | 91 |
Reverse primer TCAGGCAGCTCATAGCTCTTC | ||
GAPDH | Forward primer TTCCCGTTCAGCTCTGGG | 59 |
Reverse primer CCCTGCATCCACTGGTGC |