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. Author manuscript; available in PMC: 2022 Feb 15.
Published in final edited form as: Neuropharmacology. 2020 Nov 18;184:108408. doi: 10.1016/j.neuropharm.2020.108408

Figure 4. PKA activity is required for potentiation of DSFs and OA by 5-HT.

Figure 4.

Rat DRG neurons were treated with 10 μM H-89, 1 μM myr-PKI 14–22, or vehicle before and during exposure to 100–300 nM 5-HT. (A) Effects of H-89 and PKI on the incidence of OA during 5-HT exposure. The occurrence of OA was determined from ≥ 60-s recordings at RMP then 30-s recordings at ~−45 mV. * p < 0.025 (after Bonferroni correction for 2 comparisons). (B-D) Effects of H-89 and PKI on the frequency of medium- (3–5 mV) and large-amplitude (>5 mV) DSFs during 30-s depolarization to −45 mV (B), AP voltage threshold (C), and rheobase (D) in the presence of 5-HT. Mean ± SEM. ** Dunn’s adjusted p value < 0.01.