Patch pipettes contained 100 μM 8-Br-cAMP or vehicle in the ICS. (A) Effect of 8-Br-cAMP on the incidence of OA. The occurrence of OA was measured during ≥60-s recording at RMP then during 30-s depolarization to −45 mV. * p < 0.05. (B-E) Effect of 8-Br-cAMP on the frequency of medium- (3–5 mV) and large-amplitude (>5 mV) DSFs during 30-s depolarization to −45 mV (B), AP threshold (C), rheobase (D), and RMP (E). Mean ± SEM. * p < 0.05, ** p < 0.01, *** p < 0.001.