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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2020 Nov 3;152:106505. doi: 10.1016/j.prostaglandins.2020.106505

Table 2.

Thermal cycler program optimized for virulence gene expression qRT-PCR.

STEP NAME Temp (°C) Duration (sec) Number of Cycles
1 Initiation 95 120 1
2 DNA polymerase activation 95 20 1
3 Denaturation 95 3 50
4 Anneal and Extend 60 30 50
5 Endpoint 60 120 1