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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Cytokine. 2020 Nov 2;138:155357. doi: 10.1016/j.cyto.2020.155357

Figure 1.

Figure 1.

IL-23 is induced by TNFα and IL-17A in human primary keratinocytes and enhanced in psoriatic keratinocytes. A) IL23p19 induction by TNFα and IL-17A in human primary keratinocytes. Human primary keratinocytes were isolated from non-psoriatic individuals and cultured in CnT-07 medium. After treating the keratinocytes with cytokines as indicated for 24 hours, the total RNA was extracted for qRT-PCR analysis of IL-23p19 mRNA. B) ELISA quantification of IL-23 protein in the supernatant of human primary keratinocytes culture treated with indicated cytokines. C) IL-23 induction is enhanced in psoriatic keratinocytes. Keratinocytes were isolated from the lesional skin of patients with psoriasis (n=6) and from non-psoriatic individuals (n=6). After treating the keratinocytes with TNFα and IL-17A for 24 hours, the total RNA was extracted for qRT-PCR analysis of IL-23p19 mRNA, and D) IL23p40 mRNA. E) ELISA quantification of IL-23 protein in the culture supernatants of primary keratinocytes from patients with or without psoriasis.