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. Author manuscript; available in PMC: 2021 Mar 1.
Published in final edited form as: Glia. 2020 Oct 24;69(3):792–811. doi: 10.1002/glia.23930

FIGURE 4.

FIGURE 4

The effects of guanidine compounds on oligodendrocyte differentiation in primary oligodendrocyte precursor cells (OPCs). (a) Immunofluorescence labeling for the mature oligodendrocyte antigen O1 (red) in control rat primary OPCs grown in the presence of 5 ng/ml PDGF AA (control), in the presence of 5 ng/ml PDGF AA and 400 μM T3 (T3), or in the presence of the indicated guanidine compounds at 10 μ Blue represents DAPI. Scale, 100 μm. (b) The percentage of DAPI+ cells grown in the presence of the indicated compounds that were O1+. *, p < .05. (c) Reverse transcription-quantitative PCR (qPCR) for Pdgfra, NG2 (Cspg4), and Mbp after treatment with 5 ng/ml PDGF AA alone (control) or with 400 ng/ml T3 or 10 μM 39D11. mRNA levels are shown relative to dimethyl sulfoxide (DMSO)-treated control and plotted on a log2 scale. *, p < .05 compared to control, two-way analysis of variance (ANOVA), Tukey’s test