Skip to main content
. Author manuscript; available in PMC: 2022 Jan 21.
Published in final edited form as: Cell. 2020 Dec 30;184(2):507–520.e16. doi: 10.1016/j.cell.2020.11.048

Figure 2. Identification of sexually monomorphic and dimorphic aggression-promoting cell types.

Figure 2.

(A) “Enhancer-bashing” strategy to identify GAL4 drivers for R60G08 subpopulations.

(B) Neurons labeled by Eb5-Gal4 (attp40) in males (i-ii) and females (iii-iv). Areas outlined by dashed boxes in (i) and (iii) are enlarged in (ii) and (iv), respectively. Arrowheads: cell body locations. Scale bar: 50μm. See also Figures S3A and S4B.

(C) Activation of Eb5 neurons strongly promotes male (i-ii) and female aggression (iii-iv). Shown are bouts of approach (i and iii), lunging (ii) and headbutting (iv). Light and dark color lines: individual data and the mean, respectively. See also Figure S3B.

(D) Morphology of the sexually shared CAP neurons in males and females. Neuronal traces labelled by activated photo-activatable (PA) GFP (i-ii), CsChrimson expression driven by CAP driver (iii-iv; Eb5-Gal4 (attp40); R22F05-Gal80(attp2)), and overlay between sexes (v). Scale bar: 50μm. See also Figure S3C–E.

(E) Morphology of the male-specific MAP neurons in males. Neuronal traces labelled by activated PA-GFP (i), CsChrimson expression driven by pC1 driver (ii; NP2631/dsx-Flp), or by MAP driver (iii; Eb5-AD(vk27), R22F05-DBD(attp2)) and overlay between pC1 and MAP (iv). Scale bar: 50μm. See also Figure S4A–D.